Clarke made five appearances in all competitions for the Cod Army last season.
“Macclesfield Town are delighted to welcome 20-year-old left-back Eddie Clarke to the club, after he agreed a season-long loan deal from League One side Fleetwood Town,” the Silkmen announced on their official website.
Clarke began his career at Tranmere and made a total of 16 first-team appearances before moving on to Fleetwood in the summer of 2018.
The Silkmen have also brought in centre-back Fraser Horsfall on a one-year deal.
The 22-year-old, who came through the youth ranks at Huddersfield, joins having recently left Kidderminster.